GoTaq qPCR Master Mix from Promega

Product Specs
ItemGoTaq qPCR Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6001
Quantity5ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Construction and validation of a novel lysosomal signature for hepatocellular carcinoma prognosis, diagnosis, and therapeutic decision-makingSci RepDecember 18, 2023Jianlin Chen, Gan Gao, Yufang He, Yi Zhang, Haixia Wu, Peng Dai, Qingzhu Zheng, Hengbin Huang, Jiamiao Weng, Yue Zheng, et al.manual. cDNA was synthesized by MCE RT Master Mix for qPCR II (MCEs, NJ, USA). A GoTaq® qPCR Master Mix (A6001, Promega) was used for qPCR. Primers were synthesized by Shangya Biotechnology (Fuzhou, China). CLN3:

(2) Gadolinium Oxide Nanoparticles Reinforce the Fractionated Radiotherapy-Induced Immune Response in Tri-Negative Breast Cancer via cGAS-STING PathwayInt J NanomedicineDecember 15, 2023Boyi Yu, Xuanyi Lu, Xianglong Feng, Ting Zhao, Jiaxin Li, Yudie Lu, Fei Ye, Xiongxiong Liu, Xiaogang Zheng, Zheyu Shen, et al.transcribed cDNA. Finally, PCR was performed using a PCR kit (GoTaq qPCR Master Mix: Promega, Cat. No. A6002), PCR reaction was carried out according to the operation steps to detect the expression of related

(3) Establishment of a prognosis prediction model for lung squamous cell carcinoma related to PET/CT: basing on immunogenic cell death-related lncRNABMC Pulm MedDecember 15, 2023Yu Han, Zhiqiang Dong, Yu Xing, Yingying Zhan, Jinhai Zou, Xiaodong WangTaraka, Japan). A 20 ul RT-qPCR system was prepared following the instructions of a qPCR master mix kit (A6001, Promega, USA). The PCR primer sequences for lncRNA are provided in Supplement Table 1. Positron emission

(4) Kinin B1 receptor controls maternal adiponectin levels and influences offspring weight gainiScienceDecember 15, 2023Thaís Alves-Silva, Talita G.R. Húngaro, Leandro C. Freitas-Lima, Gabriel de Melo Arthur, Adriano C. Arruda, Raisa B. Santos, Lila M. Oyama, Marcelo A.S. Mori, Michael Bader, Ronaldo C. Araujousing 20ng of cDNA with 10μM of the primers described in Table 1 and GoTaq® qPCR Master Mix, ROX (#A6002, Promega, Madison, WI, USA), 10 μL reaction volume in QuantStudio5 equipment (Applied Biosystems). Relative

(5) A versatile method for the identification of senolytic compoundsCell StressDecember 11, 2023Chiara Annunziata, Francesca Castoldi, Schlegel Jan, Hazel X. Ang, Mina Ristovska, Stefania Melini, Robert Welch, Christian G. Riedel, Federico Pietrocolaaccording to manufacturer instructions. Real-time PCR was performed with GoTaq PCR Master Mix (Promega, #A6002) for the following genes using the stated primers: Senescence Associated Beta Galactosidase Assay Staining

(6) Intracellular Growth Inhibition and Host Immune Modulation of 3-Amino-1,2,4-triazole in Murine BrucellosisInt J Mol SciDecember 11, 2023Trang Thi Nguyen, Tran Xuan Ngoc Huy, Ched Nicole Turbela Aguilar, Alisha Wehdnesday Bernardo Reyes, Said Abdi Salad, Won-Gi Min, Hu-Jang Lee, Hyun-Jin Kim, John-Hwa Lee, Suk Kimassay kit (ab113581-Abcam, Cambridge, UK), Griess reagent system (G2930), and GoTaq®qPCR master mix (A6002) were purchased from Promega (Fitchburg, WI, USA). NP-40 (85124) and cell extraction buffer (FNN0011)

(7) PURPL and NEAT1 Long Non-Coding RNAs Are Modulated in Vascular Smooth Muscle Cell Replicative SenescenceBiomedicinesDecember 6, 2023Clara Rossi, Marco Venturin, Jakub Gubala, Angelisa Frasca, Alberto Corsini, Cristina Battaglia, Stefano BellostaQuantStudio 5 thermocycler (Applied Biosystems) in 384-well plates using the GoTaq qPCR Master Mix (A6002, Promega). A total of 10 μL PCR reactions were prepared containing 2 μL of reverse transcriptase product

(8) Comparative Transcriptome Analysis of Galeruca daurica Reveals Cold Tolerance MechanismsGenes (Basel)December 4, 2023Hongling Zhang, Feilong Sun, Wenbing Zhang, Xia Gao, Lei Du, Xiaopeng Yun, Yanyan Li, Ling Li, Baoping Pang, Yao Tanreference gene [33]. The qRT-PCR reaction system was performed according to the GoTaq® qPCR Master Mix (A6001, Promega, Madison, WI, USA) instructions for configuration. A qRT-PCR instrument (FTC-3000, Funglyn

(9) Two modes of gene regulation by TFL1 mediate its dual function in flowering time and shoot determinacy of ArabidopsisDevelopmentDecember 1, 2023Martina Cerise, V. S. da Silveira Falavigna, Gabriel Rodríguez-Maroto, Antoine Signol, Edouard Severing, H. Gao, Annabel van Driel, Coral Vincent, Sandra Wilkens, Francesca Romana Iacobini, et al.SuperScript IV System (Thermo Fisher Scientific). RT-PCR was performed using GoTaq qPCR MasterMix (Promega A6001) and the primers are listed in Table S2. Supplementary Material Click here for additional data file. Table

(10) Seasonal variation in telomerase activity and telomere dynamics in a hibernating rodent, the garden dormouse (Eliomys quercinus)Frontiers in PhysiologyNovember 22, 2023Carlos Galindo-Lalana, Franz Hoelzl, Sandrine Zahn, Caroline Habold, Jessica S. Cornils, Sylvain Giroud, Steve Smithin a final volume of 20 μL containing 10 μL of Promega BRYT Green GoTaq® qPCR Master Mix (Cat. Nr. A6001/2; Promega, Madison, United States). PCR conditions for cMYC were 10 min at 95°C followed by 40 cycles

(11) Genome-wide identification of notochord enhancers comprising the regulatory landscape of the brachyury locus in mouseDevelopmentNovember 15, 2023Dennis Schifferl, Manuela Scholze-Wittler, Alba Villaronga Luque, Milena Pustet, Lars Wittler, Jesse V. Veenvliet, Frederic Koch, Bernhard G. Herrmannperformed on a StepOnePlus (Thermo Fisher Scientific) system using the GoTaq qPCR premix (Promega, A6001) according to the manufacturers' recommendations. Each sample and primer combination was loaded in triplicate.

(12) Unveiling the molecular Culprit of arterial stiffness in vitamin D deficiency and obesity: Potential for novel therapeutic targetsHeliyonNovember 7, 2023Adel B. Elmoselhi, Amal Bouzid, Mohamed Seif Allah, Zeinab Ibrahim, Khuloud Bajbouj, Rebal S. Abou Assaleh, Thenmozhi Venkatachalam, Mohamed Madkour, Ruqaiyyah Siddiqui, Naveed Ahmed Khan, et al.100 ng of cDNA was utilized for the RT-PCR reactions, as well as Go Taq™ qPCR Master Mix (Cat. No. A6001; Promega, USA). Duplicate reactions for each sample with a final volume of 10 μl for each reaction were

(13) HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexesbioRxivNovember 3, 2023Alison Barrett, Manisha R. Shingare, Andreas Rechtsteiner, Tilini U. Wijeratne, Kelsie M. Rodriguez, Seth M. Rubin, Gerd A. MüllerDNA Clean & Concentrator-5 kits (Zymo Research, #77001-152). qPCR was performed with the GoTaq qPCR Master Mix (Promega, #A6001) on a Quantstudio 3 Real-Time PCR System (ThermoFisher Scientific). See Supplementary Table S1 for primer

(14) CIZ1 aggravates gastric cancer progression via mediating FBXL19-AS1 and miR-339-3pHeliyonNovember 1, 2023Houmin Wan, Lianzhen Wang, Bin Huo, Zhongpeng Qiao, Yingli ZhangRevertAid First Strand cDNA Synthesis Kit (K1622, Thermo Fisher, IL, USA). Next, qPCR was conducted via GoTaq qPCR Master Mix (A6001, Promega, Madison, WI, USA). The fold changes of genes were determined using 2−△△CT. Triplicate was

(15) Comprehensive analysis of the lysine succinylome in fish oil-treated prostate cancer cellsLife Science AllianceNovember 1, 2023Yifan Jiang, Chao He, Haokai Ye, Qianhan Xu, Xiuyuan Chen, Ying Chen, Jianying Sun, Sonia Rocha, Mu WangPromega) from 1 μg of extracted RNA. Target genes were amplified by qRT–PCR with BRYT Green Dye (Cat# A6001; Promega) binding. The cycle threshold (CT) values of targeted and housekeeping genes (tubulin) obtained

(16) NF-kB Regulation and the Chaperone System Mediate Restorative Effects of the Probiotic Lactobacillus fermentum LF31 in the Small Intestine and Cerebellum of Mice with Ethanol-Induced DamageBiology (Basel)November 1, 2023Letizia Paladino, Francesca Rappa, Rosario Barone, Filippo Macaluso, Francesco Paolo Zummo, Sabrina David, Marta Anna Szychlinska, Fabio Bucchieri, Everly Conway de Macario, Alberto J. L. Macario, et al.(Life Technologies, Carlsbad, CA, USA). qRT-PCR analysis was performed using GoTaq qPCR Master Mix (A6001, Promega Corporation). All samples were run in triplicate. A threshold cycle (CT) was observed in the

(17) Cystine/glutamate antiporter System xc- deficiency impairs insulin secretion in miceDiabetologiaNovember 1, 2023Axel de Baat, Daniel T. Meier, Leila Rachid, Adriano Fontana, Marianne Böni-Schnetzler, Marc Y. Donathquantitative real-time PCR (qPCR) of Chac1, Il1b and Slc7a11, SYBRgreen-based chemistry with GoTaq Polymerase (cat. A6002 Promega) and the ABI 7500 fast System was employed (Applied Biosystems, USA). Hprt was used as a housekeeping

(18) Cyclic fasting bolsters cholesterol biosynthesis inhibitors’ anticancer activityNature CommunicationsOctober 31, 2023Amr Khalifa, Ana Guijarro, Silvia Ravera, Nadia Bertola, Maria Pia Adorni, Bianca Papotti, Lizzia Raffaghello, Roberto Benelli, Pamela Becherini, Asmaa Namatalla, et al.QPCR reaction was conducted in 10 μl of a reaction mixture composed of GoTaq® qPCR Master Mix (Cat. #A6002, Promega, Madison, Wi, USA), 0.3 μM of each primer and DNase/RNase free water in the Real-Time PCR System

(19) Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 AxisAntioxidants (Basel)October 29, 2023Weixi Xie, Lang Deng, Miao Lin, Xiaoting Huang, Rui Qian, Dayan Xiong, Wei Liu, Siyuan TangThermo Fisher Scientific, USA). Quantitative polymerase chain reaction (Q-PCR) was performed using SYBR GREEN (Cat# A6001, Promega, Madison, WI, USA) and the Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules,

(20) HCG18, LEF1AS1 and lncCEACAM21 as biomarkers of disease severity in the peripheral blood mononuclear cells of COVID-19 patientsJournal of Translational MedicineOctober 26, 2023Simona Greco, Alisia Made’, Martina Mutoli, Lu Zhang, Santiago Nicolas Piella, Mélanie Vausort, Andrew I. Lumley, Antonio Paolo Beltrami, Prashant Kumar Srivastava, Valentina Milani, et al.169040503 and 172015016, Promega Corporation, US). cDNAs were analyzed using the GoTaq qPCR Master Mix (cat#A6001, lot# 0000569038, Promega Corporation, US) according to the manufacturer’s instructions on a StepOne

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